Reconstitution for intranasal Semax/Selank — preservation concerns

Bac water is standard. The benzyl alcohol is there specifically to keep contamination down over weeks. Preservative-free saline is for single-use or very short turnaround.

Fridge storage regardless of diluent. Room-temp nasal sprays on a desk are a bad idea for anything peptide-based.

I reconstitute smaller batches (1mg of peptide at a time into ~3mL) so I burn through them in 2-3 weeks. Fewer preservation headaches than sitting on a full 5mg vial diluted.

First time reconstituting anything intranasal — this helped, thanks.

Bac water works but I'm not convinced the benzyl alcohol concentration is high enough to actually matter for nasal spray that's getting used daily. You're introducing new bacteria every time you spray it anyway. The fridge thing is real though, room temp peptides degrade faster. Honestly the small batch approach makes the most sense if you can be bothered with it, just skip the preservation paranoia.

Bac water + fridge is the safest bet if you're keeping it around for weeks, but cardinal_rule has a point about recontamination with daily use. The benzyl alcohol in bac water tops out around 0.9%, which does suppress growth but isn't foolproof once you're introducing air and nasal flora repeatedly. Small batch rotation is honestly the move if you have the discipline for it, gets you out of the preservation game entirely. If you go full small batch though, preservative-free saline is fine since you're burning through it in like 14-21 days before anything sketchy happens.